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Human Na+ Taurocholate Cotransporting Polypeptide (NTCP) ELISA Kit

SKU:
HUDL02104
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q14973
Sensitivity:
0.27ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
SLC10A1,NTCP1,Solute Carrier Family 10 Member 1,Sodium/Bile Acid Cotransporter Family,Cell growth-inhibiting gene 29 protein,Na(+)/bile acid cotransporter
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Na+ Taurocholate Cotransporting Polypeptide (NTCP) ELISA Kit

The Human Na+ Taurocholate Cotransporting Polypeptide (NTCP) ELISA Kit is a cutting-edge tool designed for the precise measurement of NTCP levels in human samples including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring highly accurate and consistent results for a variety of research applications. NTCP is a vital transporter protein involved in the uptake of bile acids into hepatocytes, playing a crucial role in bile acid homeostasis and liver function.

Dysregulation of NTCP has been linked to various liver diseases and disorders, making it a valuable biomarker for studying liver health and potential therapeutic interventions. With the Human NTCP ELISA Kit, researchers can effectively explore the role of NTCP in liver function, bile acid metabolism, and related diseases, advancing our understanding of these complex biological processes and potentially leading to innovative treatment strategies.

Product Name:Human Na+ Taurocholate Cotransporting Polypeptide (NTCP) ELISA Kit
Product Code:HUDL02104
Size:96 Assays
Target:NTCP
Synonyms:SLC10A1, NTCP1, Solute Carrier Family 10 Member 1,Sodium/Bile Acid Cotransporter Family, Cell growth-inhibiting gene 29 protein, Na(+)/bile acid cotransporter
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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