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Human N-Methylpurine DNA Glycosylase (MPG) ELISA Kit

SKU:
HUDL01893
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P29372
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
AAG,APNG,ADPG,CRA36.1,MDG,Mid1,PIG11,PIG16,Anpg,DNA-3-Methyladenine Glycosylase,Alkyladenine DNA Glycosylase,3-alkyladenine DNA glycosylase
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Human N-Methylpurine DNA Glycosylase (MPG) ELISA Kit

The Human N-Methylpurine DNA Glycosylase (MPG) ELISA Kit is a powerful tool for the precise measurement of MPG levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it an invaluable asset for various research purposes.MPG is an essential enzyme involved in base excision repair, playing a crucial role in maintaining genomic stability and integrity. Dysregulation of MPG activity has been linked to DNA damage, mutagenesis, and cancer development, highlighting the significance of monitoring MPG levels in biological samples.

By using the Human MPG ELISA Kit, researchers can gain comprehensive insights into the role of MPG in DNA repair processes, cancer biology, and other disease conditions. This kit offers a reliable platform for studying MPG function and exploring its potential as a therapeutic target for various diseases. Unlock the potential of your research with the Human MPG ELISA Kit.

Product Name:Human N-Methylpurine DNA Glycosylase (MPG) ELISA Kit
Product Code:HUDL01893
Size:96 Assays
Target:MPG
Synonyms:AAG, APNG, ADPG, CRA36.1, MDG, Mid1, PIG11, PIG16, Anpg, DNA-3-Methyladenine Glycosylase, Alkyladenine DNA Glycosylase, 3-alkyladenine DNA glycosylase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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