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Human N-Acetyltransferase 2 (NAT2) ELISA Kit

SKU:
HUDL01988
Product Type:
ELISA Kit
Sensitivity:
0.25ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
AAC2,PNAT,Arylamine N-Acetyltransferase,Arylamide acetylase 2,Polymorphic arylamine N-acetyltransferase
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
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Description

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Human N-Acetyltransferase 2 (NAT2) ELISA Kit

The Human N-Acetyltransferase 2 (NAT2) ELISA Kit is specifically designed for the accurate measurement of NAT2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.NAT2 is an important enzyme involved in drug metabolism and detoxification processes in the body. Variations in NAT2 activity have been linked to differences in drug responses and susceptibility to certain diseases.

Therefore, measuring NAT2 levels can provide valuable insights into individual drug metabolism and potential disease risks.With its ability to accurately quantify NAT2 levels, this ELISA kit is a valuable tool for researchers studying drug metabolism, personalized medicine, and disease susceptibility. Its high performance and ease of use make it a valuable asset for laboratory investigations and drug development studies.

Product Name:Human N-Acetyltransferase 2 (NAT2) ELISA Kit
Product Code:HUDL01988
Size:96 Assays
Target:NAT2
Synonyms:AAC2, PNAT, Arylamine N-Acetyltransferase, Arylamide acetylase 2, Polymorphic arylamine N-acetyltransferase
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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