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Human N-Acetyltransferase 1 (NAT1) ELISA Kit

SKU:
HUDL01987
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P18440
Sensitivity:
0.61ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
AAC1,Arylamine N-Acetyltransferase,Arylamide acetylase 1,Monomorphic arylamine N-acetyltransferase
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human N-Acetyltransferase 1 (NAT1) ELISA Kit

The Human N-Acetyltransferase 1 (NAT1) ELISA Kit is specifically designed for the accurate and efficient detection of NAT1 levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and offers exceptional specificity, ensuring precise and consistent results for a variety of research applications.NAT1 is an important enzyme involved in the metabolism of drugs and carcinogens, making it a valuable biomarker for studying drug metabolism, cancer development, and personalized medicine approaches.

By accurately measuring NAT1 levels, researchers can gain valuable insights into the role of this enzyme in various disease processes and potential treatment strategies.With its high performance and reliability, the Human NAT1 ELISA Kit is an essential tool for researchers looking to unravel the complex mechanisms underlying drug metabolism and cancer progression. Order your kit today and advance your research with confidence.

Product Name:Human N-Acetyltransferase 1 (NAT1) ELISA Kit
Product Code:HUDL01987
Size:96 Assays
Target:NAT1
Synonyms:AAC1, Arylamine N-Acetyltransferase, Arylamide acetylase 1, Monomorphic arylamine N-acetyltransferase
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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