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Human N-Acetyl Beta-D-Glucosaminidase (NAGase) ELISA Kit

SKU:
HUDL01980
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O60502
Sensitivity:
0.55ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
MGEA5,MEA5,NCOAT,Hexosaminidase C,N-Acetylhexosaminidase,Hyaluronidase,Meningioma-Expressed Antigen 5,Nuclear Cytoplasmic O-GlcNAcase And Acetyltransferase
Reactivity:
Human
Sample Type:
Urine
€649
Frequently bought together:

Description

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Human N-Acetyl Beta-D-Glucosaminidase (NAGase) ELISA Kit

The Human N-acetyl-beta-D-glucosaminidase (NAGase) ELISA Kit is a powerful tool for researchers seeking to accurately measure NAGase levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research purposes.Nagase is an enzyme crucial for the breakdown of glycosaminoglycans in the body, playing a key role in various biological processes. Dysregulation of NAGase activity has been implicated in diseases such as lysosomal storage disorders and kidney diseases, making it an important target for biomarker research and potential therapeutic interventions.

With its easy-to-use design and robust performance, the Human NAGase ELISA Kit provides a valuable tool for studying NAGase biology and its implications in human health and disease. Trust in this kit for accurate and reproducible results in your research endeavors.

Product Name:Human N-Acetyl Beta-D-Glucosaminidase (NAGase) ELISA Kit
Product Code:HUDL01980
Size:96 Assays
Target:NAGase
Synonyms:MGEA5, MEA5, NCOAT, Hexosaminidase C, N-Acetylhexosaminidase, Hyaluronidase, Meningioma-Expressed Antigen 5, Nuclear Cytoplasmic O-GlcNAcase And Acetyltransferase
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)90-10296
EDTA plasma (n=5)90-10195
Heparin plasma (n=5)91-9894
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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