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Human Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B) ELISA Kit

SKU:
HUDL01959
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
A7E2Y1
Sensitivity:
0.069ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MYH14,MHC14,Antigen MLAA-21,Myosin cardiac muscle beta chain
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
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Description

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Human Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B) ELISA Kit

The Human Myosin Heavy Chain 7B (Cardiac Muscle Beta, MYH7B) ELISA Kit is specifically designed for the precise detection of MYH7B levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.MYH7B is a key protein involved in cardiac muscle function, playing a crucial role in muscle contraction and cardiac performance. Abnormalities in MYH7B expression have been linked to various cardiovascular conditions, making it a valuable biomarker for studying heart diseases and developing potential therapeutic interventions.

With its user-friendly design and reliable performance, the Human MYH7B ELISA Kit from Assay Genie is an indispensable tool for researchers and clinicians working in the field of cardiovascular research. Order now and unlock new possibilities in cardiac health studies.

Product Name:Human Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B) ELISA Kit
Product Code:HUDL01959
Size:96 Assays
Target:MYH7B
Synonyms:MYH14, MHC14, Antigen MLAA-21, Myosin cardiac muscle beta chain
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-10095
Heparin plasma (n=5)91-10196
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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