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Human Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) ELISA Kit

SKU:
HUDL01958
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P13533
Sensitivity:
0.125ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ASD3,MYHC,MYHCA,MyHC-alpha,Cardiomyopathy,Hypertrophic 1,Myosin heavy chain, cardiac muscle alpha isoform
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

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Human Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) ELISA Kit

The Human Myosin Heavy Chain 6 (Cardiac Muscle Alpha) ELISA Kit is specifically designed for the precise and sensitive measurement of myosin heavy chain 6 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers unmatched accuracy and reliability, ensuring consistent and reproducible results for a variety of research purposes.Myosin heavy chain 6, also known as cardiac muscle alpha, is a critical protein involved in muscle contraction and function.

Abnormal levels of myosin heavy chain 6 have been linked to various cardiac diseases, making it a key biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human Myosin Heavy Chain 6 (Cardiac Muscle Alpha) ELISA Kit is an essential tool for researchers in the fields of cardiology, molecular biology, and medicine, providing valuable insights into the role of myosin heavy chain 6 in cardiac health and disease.

Product Name:Human Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6) ELISA Kit
Product Code:HUDL01958
Size:96 Assays
Target:MYH6
Synonyms:ASD3, MYHC, MYHCA, MyHC-alpha, Cardiomyopathy,Hypertrophic 1, Myosin heavy chain, cardiac muscle alpha isoform
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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