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Human Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3) ELISA Kit

SKU:
HUDL01956
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11055
Sensitivity:
0.126ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
HEMHC,MYHC-EMB,MYHSE1,SMHCE,Myosin,Skeletal,Heavy chain,Embryonic 1,Myosin heavy chain, fast skeletal muscle, embryonic
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3) ELISA Kit

The Human Myosin Heavy Chain 3 (Skeletal Muscle Embryonic) ELISA Kit from Assay Genie is a cutting-edge tool for measuring levels of MYH3 in human serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit provides reliable and reproducible results, making it an invaluable resource for a variety of research applications.MYH3 is an important protein involved in skeletal muscle development and function. Its expression is critical for muscle contraction and movement.

Dysregulation of MYH3 has been associated with various muscle disorders and diseases, making it a key biomarker for studying muscle development and degenerative conditions.Whether you are studying muscle physiology, developmental biology, or disease mechanisms, the Human Myosin Heavy Chain 3 ELISA Kit from Assay Genie is the perfect tool for accurate and precise measurement of MYH3 levels. Trust Assay Genie for high-quality research tools to advance your scientific discoveries.

Product Name:Human Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3) ELISA Kit
Product Code:HUDL01956
Size:96 Assays
Target:MYH3
Synonyms:HEMHC, MYHC-EMB, MYHSE1, SMHCE, Myosin,Skeletal,Heavy chain,Embryonic 1, Myosin heavy chain, fast skeletal muscle, embryonic
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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