The Myoglobin (MYO) ELISA Kit is specifically designed for the accurate quantitative measurement of myoglobin levels in human samples. Myoglobin, a heme protein found in skeletal and cardiac muscle, serves as an essential biomarker for detecting muscle injury and cardiac stress. Elevated levels of myoglobin in the blood are indicative of muscle damage, such as in cases of myocardial infarction or skeletal muscle trauma. This ELISA kit offers exceptional sensitivity and specificity, enabling precise and reproducible results in the quantification of myoglobin across various biological samples.
Manufactured under strict quality control processes, the MYO ELISA Kit ensures reliable performance and user-friendly operation, making it suitable for both research and clinical applications. Trust in the MYO ELISA Kit from Assay Genie for precise and dependable analysis of this critical biomarker in your experimental or diagnostic studies.
Product Name:
Human MYO (Myoglobin) ELISA Kit
SKU:
AEES00060
Target:
Human MYO (Myoglobin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MYO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MYO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MYO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MYO. You can calculate the concentration of Human MYO in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
90-101
97-105
Average (%)
100
93
100
1:4
Range (%)
99-106
95-109
99-109
Average (%)
102
103
104
1:8
Range (%)
100-111
93-104
94-105
Average (%)
105
96
102
1:16
Range (%)
94-105
87-96
86-99
Average (%)
99
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
98
EDTA plasma (n=5)
96-108
100
Cell culture media (n=5)
96-104
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.86
2.33
9.08
0.98
2.3
8.81
Standard deviation
0.04
0.11
0.47
0.05
0.11
0.39
C V (%)
4.65
4.72
5.18
5.1
4.78
4.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MYO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MYO in samples. No significant cross-reactivity or interference between Human MYO and analogues was observed.
