Human MYL5 Recombinant Protein (RPPB4035)
- SKU:
- RPPB4035
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q02045
Description
| Product Name: | Human MYL5 Recombinant Protein |
| Product Code: | RPPB4035 |
| Size: | 20µg |
| Species: | Human |
| Target: | MYL5 |
| Synonyms: | Myosin light chain 5, Myosin regulatory light chain 5, Superfast myosin regulatory light chain 2, MYLC2, MyLC-2, MYL5. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | MYL5 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMDQNRD GFIDKEDLKD TYASLGKTNV KDDELDAMLK EASGPINFTM FLNLFGEKLS GTDAEETILN AFKMLDPDGK GKINKEYIKR LLMSQADKMT AEEVDQMFQF ASIDVAGNLD YKALSYVITH GEEKEE |
Myosin regulatory light chain 5 (MYL5) is a hexameric ATPase cellular motor protein. Myosin is comprised of 2 heavy chains, 2 nonphosphorylatable alkali light chains, and 2 phosphorylatable regulatory light chains. MYL5 is a regulatory light chain and is expressed in the fetal muscle and in the adult retina, cerebellum, and basal ganglia. The reconstitution of myosin with MYL5 or alkali light chain increases filament velocity to intermediate rates, and the re-addition of both classes of light chains fully reinstates the original sliding pace.
MYL5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 156 amino acids (1-132 a.a.) and having a molecular mass of 17.4kDa.MYL5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MYL5: 2 isoforms of the human protein are produced by alternative splicing.Protein type: Motility/polarity/chemotaxis; ContractileChromosomal Location of Human Ortholog: 4p16.3Cellular Component: muscle myosin complexMolecular Function: structural constituent of muscle; calcium ion bindingBiological Process: regulation of muscle contraction |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes one of the myosin light chains, a component of the hexameric ATPase cellular motor protein myosin. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. This gene product, one of the regulatory light chains, is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q02045 |
| NCBI GenInfo Identifier: | 4505305 |
| NCBI Gene ID: | 4636 |
| NCBI Accession: | NP_002468.1 |
| UniProt Secondary Accession: | Q02045,Q8IXL8 |
| UniProt Related Accession: | Q02045 |
| Molecular Weight: | 19,534 Da |
| NCBI Full Name: | myosin light chain 5 |
| NCBI Synonym Full Names: | myosin, light chain 5, regulatory |
| NCBI Official Symbol: | MYL5�� |
| NCBI Official Synonym Symbols: | |
| NCBI Protein Information: | myosin light chain 5; MYLC2; myLC-2; myosin regulatory light chain 5; myosin, light polypeptide 5, regulatory; superfast myosin regulatory light chain 2 |
| UniProt Protein Name: | Myosin light chain 5 |
| UniProt Synonym Protein Names: | Myosin regulatory light chain 5; Superfast myosin regulatory light chain 2; MYLC2; MyLC-2 |
| Protein Family: | Myosin |
| UniProt Gene Name: | MYL5�� |
| UniProt Entry Name: | MYL5_HUMAN |
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