Human MYH9 (Myosin Heavy Chain 9, Non Muscle) CLIA Kit
The Human MYH9 (Myosin Heavy Chain 9 Non-Muscle) CLIA Kit is a reliable and accurate tool for the quantification of MYH9 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.MYH9 is a non-muscle myosin protein that plays a crucial role in cell motility, cytokinesis, and organelle transport. Mutations in the MYH9 gene have been linked to several genetic disorders, including MYH9-related diseases and syndromes.
Therefore, measuring MYH9 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic strategies.Overall, the Human MYH9 CLIA Kit is a valuable tool for researchers interested in studying MYH9-related diseases, understanding its molecular functions, and exploring its potential as a biomarker for diagnostic and therapeutic purposes.
Product Name:
Human MYH9 (Myosin Heavy Chain 9, Non Muscle) CLIA Kit
SKU:
HUES00457
Target:
Human MYH9 (Myosin Heavy Chain 9, Non Muscle)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MYH9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MYH9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MYH9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MYH9. You can calculate the concentration of Human MYH9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
90-101
87-99
Average (%)
94
96
93
1:4
Range (%)
88-101
87-102
88-99
Average (%)
93
94
93
1:8
Range (%)
96-107
92-105
104-117
Average (%)
101
100
110
1:16
Range (%)
92-110
101-114
86-101
Average (%)
100
108
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
104
EDTA plasma (n=5)
99-114
107
Cell culture media (n=5)
85-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.11
253.63
828.04
92.51
258.3
785.16
Standard deviation
12.89
28.51
84.29
7.65
25.98
82.99
C V (%)
12.62
11.24
10.18
8.27
10.06
10.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MYH9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MYH9 in samples. No significant cross-reactivity or interference between Human MYH9 and analogues was observed.
