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Human Myelin Oligodendrocyte Glycoprotein (MOG) ELISA Kit

SKU:
HUDL01891
Product Type:
ELISA Kit
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

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Human Myelin Oligodendrocyte Glycoprotein (MOG) ELISA Kit

The Human Myelin Oligodendrocyte Glycoprotein (MOG) ELISA Kit is specifically designed for the precise measurement of MOG levels in human biological samples including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and accuracy, ensuring dependable and consistent results, making it perfect for various research purposes.MOG is a key protein associated with myelin, which is essential for the proper functioning of the nervous system.

Abnormal levels of MOG have been linked to autoimmune diseases such as multiple sclerosis, making it a crucial biomarker for studying these conditions and potentially developing new treatments. With the Human MOG ELISA Kit, researchers can easily assess MOG levels and further understand its role in neurological disorders.

Product Name:Human Myelin Oligodendrocyte Glycoprotein (MOG) ELISA Kit
Product Code:HUDL01891
Size:96 Assays
Target:MOG
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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