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Human Myelin Associated Glycoprotein (MAG) ELISA Kit

SKU:
HUDL01780
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P20916
Sensitivity:
0.113ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
GMA,S-MAG,Siglec-4,SIGLEC4A,Sialic Acid Binding Ig-Like Lectin 4A
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Myelin Associated Glycoprotein (MAG) ELISA Kit

The Human Myelin-Associated Glycoprotein (MAG) ELISA Kit is specifically designed for the precise detection of MAG levels in human serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, guaranteeing dependable and consistent results, which makes it an excellent choice for a variety of research applications.MAG is a key protein involved in the formation and maintenance of myelin, a crucial component of the nervous system.

Dysregulation of MAG has been linked to neurological disorders such as multiple sclerosis and peripheral neuropathy. By accurately measuring MAG levels, researchers can gain valuable insights into the pathophysiology of these conditions and potentially identify new therapeutic targets.

Product Name:Human Myelin Associated Glycoprotein (MAG) ELISA Kit
Product Code:HUDL01780
Size:96 Assays
Target:MAG
Synonyms:GMA, S-MAG, Siglec-4, SIGLEC4A, Sialic Acid Binding Ig-Like Lectin 4A
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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