Human MYC (V-Myc Myelocytomatosis Viral Oncogene Homolog) CLIA Kit
The Human MYC (v-Myc Myelocytomatosis Viral Oncogene Homolog) CLIA Kit is a cutting-edge assay designed for the precise measurement of MYC levels in human samples including serum, plasma, and cell culture supernatants. This advanced kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a wide array of research endeavors.The MYC protein, also known as the v-Myc myelocytomatosis viral oncogene homolog, is a critical regulator of cell growth and proliferation. Its abnormal expression is frequently associated with tumorigenesis and cancer progression, making it a crucial target for therapeutic intervention and biomarker analysis.
By utilizing the Human MYC CLIA Kit, researchers can gain valuable insights into the role of MYC in cancer biology, uncovering new avenues for personalized treatment strategies and diagnostic applications. This innovative kit is a must-have tool for any laboratory seeking to unravel the complexities of oncogene signaling pathways and unlock the potential for novel cancer therapies.
Product Name:
Human MYC (V-Myc Myelocytomatosis Viral Oncogene Homolog) CLIA Kit
SKU:
HUES00518
Target:
Human MYC (V-Myc Myelocytomatosis Viral Oncogene Homolog)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MYC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MYC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MYC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MYC. You can calculate the concentration of Human MYC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-120
99-117
93-109
Average (%)
110
108
100
1:4
Range (%)
87-102
102-116
95-107
Average (%)
94
109
101
1:8
Range (%)
96-112
96-110
99-113
Average (%)
103
103
105
1:16
Range (%)
89-99
88-102
85-96
Average (%)
94
96
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
105
EDTA plasma (n=5)
89-103
94
Cell culture media (n=5)
91-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.79
276.13
918.81
104.29
252.58
950.51
Standard deviation
9.61
22.86
68.64
12.4
23.24
97.71
C V (%)
9.44
8.28
7.47
11.89
9.2
10.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MYC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MYC in samples. No significant cross-reactivity or interference between Human MYC and analogues was observed.
