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Human MYBPC3 Recombinant Protein (RPPB4027)

SKU:
RPPB4027
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q14896
€309
Frequently bought together:

Description

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Product Name:Human MYBPC3 Recombinant Protein
Product Code:RPPB4027
Size:10µg
Species:Human
Target:MYBPC3
Synonyms:Myosin Binding Protein C Cardiac, C-Protein Cardiac Muscle Isoform, Myosin-Binding Protein C Cardiac, Cardiac MyBP-C, CMD1MM, LVNC10, MYBP-C, CMH4, FHC, MYBPC3.
Source:Escherichia Coli
Physical Appearance:Filtered White lyophilized (freeze-dried) powder.
Formulation:MYBPC3 was filtered (0.4�m) and lyophilized in 20mM Tris buffer and 50mM NaCl, pH 7.5.
Solubility:It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. MYBPC3 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Stability:Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MKHHHHHHASMPEPGKKPVS AFSKKPRSVE VAAGSPAVFE AETERAGVKV RWQRGGSDIS ASNKYGLATE GTRHTLTVRE VGPADQGSYA VIAGSSKVKF DLKVIEAEKA EPMLAPAPAP AEATGAPGEA PAPAAELGES APSPKGSSSA ALNGPTPGAP DDPIGLFVMR PQDGEVTVGG SITFSARVAG ASLLKPPVVK WFKGKWVDLS SKVGQHLQLH DSYDRASKVY LFELHITDAQ PAFTGSYRCE VSTKDKFDCS NFNLTVHEAM GTGDLDLLSA F

Myosin Binding Protein C, Cardiac (MYBPC3) is the cardiac isoform of myosin-binding protein C expressed exclusively in heart muscle. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. Regulatory phosphorylation of the cardiac isoform in vivo by cAMP-dependent protein kinase upon adrenergic stimulation may be associated with modulation of cardiac contraction. MYBPC3 gene mutations are one of the causes of familial hypertrophic cardiomyopathy. In vitro MYBPC3 binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. MYBPC3 may modulate muscle contraction or it may have a more structural role.

MYBPC3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Met1-Phe271) containing 281 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 29.6kDa.

UniProt Protein Function:MYBPC3: Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role. Belongs to the immunoglobulin superfamily. MyBP family.
UniProt Protein Details:

Protein type:Myosin-binding

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: A band; cytosol; sarcomere; striated muscle thick filament

Molecular Function:ATPase activator activity; identical protein binding; myosin binding; myosin heavy chain binding; protein binding; structural constituent of muscle

Biological Process: cardiac muscle contraction; heart morphogenesis; muscle filament sliding; positive regulation of ATPase activity; regulation of muscle filament sliding; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis

Disease: Cardiomyopathy, Familial Hypertrophic, 4; Left Ventricular Noncompaction 10

NCBI Summary:MYBPC3 encodes the cardiac isoform of myosin-binding protein C. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. MYBPC3, the cardiac isoform, is expressed exclussively in heart muscle. Regulatory phosphorylation of the cardiac isoform in vivo by cAMP-dependent protein kinase (PKA) upon adrenergic stimulation may be linked to modulation of cardiac contraction. Mutations in MYBPC3 are one cause of familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
UniProt Code:Q14896
NCBI GenInfo Identifier:425906074
NCBI Gene ID:4607
NCBI Accession:Q14896.4
UniProt Secondary Accession:Q14896,Q16410, Q6R2F7, Q9UE27, Q9UM53, A5PL00,
UniProt Related Accession:Q14896
Molecular Weight:140,703 Da
NCBI Full Name:Myosin-binding protein C, cardiac-type
NCBI Synonym Full Names:myosin binding protein C, cardiac
NCBI Official Symbol:MYBPC3��
NCBI Official Synonym Symbols:FHC; CMH4; CMD1MM; LVNC10; MYBP-C��
NCBI Protein Information:myosin-binding protein C, cardiac-type
UniProt Protein Name:Myosin-binding protein C, cardiac-type
UniProt Synonym Protein Names:C-protein, cardiac muscle isoform
Protein Family:Myosin-binding protein
UniProt Gene Name:MYBPC3��
UniProt Entry Name:MYPC3_HUMAN

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