Human MUSK (Muscle Skeletal Receptor Tyrosine Kinase) ELISA Kit
The Human MUSK (Muscle-Skeletal Receptor Tyrosine Kinase) ELISA Kit is specifically designed for the sensitive and accurate detection of MUSK levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.MUSK is a critical protein involved in neuromuscular junction formation and maintenance, playing a vital role in muscle development and function.
Dysregulation of MUSK has been associated with various neuromuscular diseases, making it a valuable biomarker for studying these conditions and developing potential therapies.Overall, the Human MUSK ELISA Kit provides researchers with a reliable tool for investigating the role of MUSK in neuromuscular disorders and exploring potential treatment options.
Product Name:
Human MUSK (Muscle Skeletal Receptor Tyrosine Kinase) ELISA Kit
SKU:
HUES02391
Target:
Human MUSK (Muscle Skeletal Receptor Tyrosine Kinase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MUSK. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUSK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUSK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MUSK. You can calculate the concentration of Human MUSK in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
96-112
87-101
Average (%)
95
103
94
1:4
Range (%)
88-102
82-93
81-93
Average (%)
94
87
87
1:8
Range (%)
94-110
83-94
87-98
Average (%)
100
88
92
1:16
Range (%)
91-108
83-93
84-98
Average (%)
99
88
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
84-98
91
Cell culture media (n=5)
94-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.38
3.97
0.55
1.38
3.96
Standard deviation
0.04
0.07
0.2
0.04
0.07
0.18
C V (%)
7.27
5.07
5.04
7.27
5.07
4.55
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MUSK concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MUSK in samples. No significant cross-reactivity or interference between Human MUSK and analogues was observed.
