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Human Multimerin 1 (MMRN1) ELISA Kit

SKU:
HUDL01888
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q13201
Sensitivity:
9.6pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
ECM,EMILIN4,GPIa,MMRN,Glycoprotein Ia,Elastin microfibril interface located protein 4,Endothelial cell multimerin,Platelet glycoprotein Ia,155 kDa platelet multimerin
Reactivity:
Human
Sample Type:
Serum, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Multimerin 1 (MMRN1) ELISA Kit

The Human Multimerin-1 (MMRN1) ELISA Kit is a cutting-edge tool for the precise measurement of Multimerin-1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research applications.Multimerin-1 is a key protein involved in various biological processes, particularly in blood coagulation and platelet function. Its dysregulation has been linked to several health conditions including cardiovascular diseases and thrombosis.

Therefore, the Human Multimerin-1 ELISA Kit is an indispensable resource for investigating the role of Multimerin-1 in disease pathogenesis and potential therapeutic strategies.Overall, the Human Multimerin-1 ELISA Kit provides researchers with a reliable and efficient method for studying Multimerin-1 levels and its implications in human health and disease.

Product Name:Human Multimerin 1 (MMRN1) ELISA Kit
Product Code:HUDL01888
Size:96 Assays
Target:MMRN1
Synonyms:ECM, EMILIN4, GPIa, MMRN, Glycoprotein Ia, Elastin microfibril interface located protein 4, Endothelial cell multimerin, Platelet glycoprotein Ia, 155 kDa platelet multimerin
Detection Method:Sandwich
Range:31.25-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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