The Human MUC7 (Mucin 7) ELISA Kit is specifically designed for the quantitative measurement of MUC7 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Mucin 7 is a vital protein involved in mucosal defense and oral health, playing a crucial role in protecting the mucosal surfaces and maintaining a healthy oral cavity.
Abnormalities in MUC7 levels have been linked to conditions such as periodontal disease and respiratory infections, making it a valuable biomarker for studying these diseases and developing targeted therapies.With its reliable performance and ease of use, the Human MUC7 ELISA Kit is a valuable tool for researchers and healthcare professionals looking to investigate mucin 7 levels and their implications in human health and disease.
Product Name:
Human MUC7 (Mucin 7) ELISA Kit
SKU:
HUES03164
Target:
Human MUC7 (Mucin 7)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MUC7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MUC7. You can calculate the concentration of Human MUC7 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
88-100
87-103
Average (%)
102
95
94
1:4
Range (%)
88-101
82-95
82-96
Average (%)
95
87
88
1:8
Range (%)
91-103
79-92
87-102
Average (%)
98
85
94
1:16
Range (%)
91-104
84-96
83-94
Average (%)
97
89
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
89-103
96
Cell culture media (n=5)
87-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.1
2.41
8.67
1.14
2.44
8.9
Standard deviation
0.06
0.11
0.36
0.06
0.14
0.29
C V (%)
5.45
4.56
4.15
5.26
5.74
3.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MUC7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MUC7 in samples. No significant cross-reactivity or interference between Human MUC7 and analogues was observed.
