The Human MUC7 (Mucin 7) CLIA Kit is specifically designed for the quantitative measurement of Mucin 7 levels in human samples such as serum, plasma, and cell culture supernatants. With a high degree of sensitivity and precision, this kit delivers accurate and reproducible results, making it an invaluable tool for various research applications.Mucin 7 is a vital glycoprotein that plays a crucial role in maintaining mucosal integrity and protecting against microbial infections. Dysregulation of Mucin 7 expression has been linked to various diseases, including respiratory disorders, gastrointestinal illnesses, and oral health issues.
Therefore, the ability to accurately measure Mucin 7 levels can provide valuable insights into these conditions and facilitate the development of targeted therapeutic strategies.Overall, the Human MUC7 CLIA Kit offers researchers a reliable and efficient solution for studying the role of Mucin 7 in health and disease, ultimately advancing our understanding of mucosal biology and potential treatment options.
Product Name:
Human MUC7 (Mucin 7) CLIA Kit
SKU:
HUES01189
Target:
Human MUC7 (Mucin 7)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MUC7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MUC7. You can calculate the concentration of Human MUC7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
103-118
102-116
Average (%)
97
109
109
1:4
Range (%)
92-108
87-102
94-109
Average (%)
99
94
102
1:8
Range (%)
100-113
90-103
102-116
Average (%)
106
97
108
1:16
Range (%)
94-110
98-111
91-103
Average (%)
100
105
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
103
EDTA plasma (n=5)
86-101
92
Cell culture media (n=5)
90-108
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
184.17
459.22
1507.94
181.42
447.15
1534.2
Standard deviation
19.1
39.03
163.61
17.6
32.73
93.89
C V (%)
10.37
8.5
10.85
9.7
7.32
6.12
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MUC7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MUC7 in samples. No significant cross-reactivity or interference between Human MUC7 and analogues was observed.
