The Human MUC21 (Mucin 21) CLIA Kit is a cutting-edge assay designed for the precise measurement of MUC21 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results that are crucial for various research applications.MUC21 is a key protein involved in mucin production, playing a vital role in maintaining mucosal barrier function in the body. Dysregulation of MUC21 expression has been linked to various inflammatory and respiratory diseases, making it a valuable biomarker for studying these conditions and developing targeted therapies.
With its advanced technology and reliable performance, the Human MUC21 CLIA Kit is a valuable tool for researchers and clinicians seeking to further understand the role of MUC21 in health and disease. Order now to advance your research efforts and uncover new insights into mucin biology.
Product Name:
Human MUC21 (Mucin 21) CLIA Kit
SKU:
HUES00447
Target:
Human MUC21 (Mucin 21)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MUC21. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC21 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC21, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MUC21. You can calculate the concentration of Human MUC21 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-120
99-113
90-105
Average (%)
109
104
97
1:4
Range (%)
94-107
103-116
86-99
Average (%)
100
110
93
1:8
Range (%)
90-103
91-103
90-102
Average (%)
97
98
96
1:16
Range (%)
96-110
100-114
87-102
Average (%)
104
108
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-103
94
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
94-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.37
317.37
892.17
95.71
316.23
868.62
Standard deviation
12.77
34.12
97.87
7.83
34.88
56.29
C V (%)
12.85
10.75
10.97
8.18
11.03
6.48
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MUC21 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MUC21 in samples. No significant cross-reactivity or interference between Human MUC21 and analogues was observed.
