The Human MUC17 (Mucin 17) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Mucin 17 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for various research applications.Mucin 17 is a mucin protein that plays a crucial role in mucosal barrier function and immune response. Abnormal expression of Mucin 17 has been associated with various diseases, including inflammatory bowel disease, colorectal cancer, and gastric cancer.
Therefore, studying Mucin 17 levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of potential diagnostic and therapeutic strategies.Overall, the Human MUC17 CLIA Kit is a valuable tool for researchers and healthcare professionals looking to investigate the role of Mucin 17 in health and disease. Its high sensitivity and specificity make it a reliable choice for accurate measurement of Mucin 17 levels in human samples.
Product Name:
Human MUC17 (Mucin 17) CLIA Kit
SKU:
HUES00417
Target:
Human MUC17 (Mucin 17)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MUC17. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC17 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC17, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MUC17. You can calculate the concentration of Human MUC17 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
92-105
101-118
Average (%)
102
99
107
1:4
Range (%)
104-116
86-99
95-112
Average (%)
110
92
103
1:8
Range (%)
104-116
86-100
89-102
Average (%)
110
92
94
1:16
Range (%)
102-120
93-108
86-102
Average (%)
109
99
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-113
103
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
90-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.25
127.99
322.69
39.11
122.09
354.35
Standard deviation
4.1
9.07
28.11
4.02
11.01
26.9
C V (%)
9.94
7.09
8.71
10.28
9.02
7.59
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MUC17 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MUC17 in samples. No significant cross-reactivity or interference between Human MUC17 and analogues was observed.
