Human Mucin 13, Cell Surface Associated (MUC13) ELISA Kit
The Human MUC13 (Mucin 13) Cell Surface Associated ELISA Kit is specifically designed for the accurate detection of MUC13 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.MUC13 is a key protein involved in cell signaling and adhesion, playing an important role in various biological processes. Its dysregulation has been linked to several diseases, including cancer, inflammatory bowel disease, and respiratory disorders.
As a result, MUC13 serves as a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With its advanced technology and user-friendly protocol, the Human MUC13 ELISA Kit is a valuable tool for researchers seeking to investigate the role of MUC13 in disease pathogenesis and develop targeted treatments. Trust in the accuracy and reliability of this kit for your research needs.
Product Name:
Human Mucin 13, Cell Surface Associated (MUC13) ELISA Kit
Product Code:
HUDL01928
Size:
96 Assays
Target:
MUC13
Synonyms:
DRCC1, RECC, Down-Regulated In Colon Cancer 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
