The Human MUC1 (Mucin 1) CLIA Kit is a powerful tool for the precise measurement of MUC1 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it suitable for various research applications.MUC1, also known as mucin 1, is a glycoprotein involved in cell adhesion and signaling. Abnormal expression of MUC1 has been linked to various diseases, including cancer, inflammation, and autoimmune disorders.
As a key biomarker, studying MUC1 levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of potential therapeutic strategies.Overall, the Human MUC1 CLIA Kit offers researchers a reliable and efficient solution for the quantitative analysis of MUC1 levels, facilitating advancements in the field of biomedical research.
Product Name:
Human MUC1 (Mucin 1) CLIA Kit
SKU:
HUES00408
Target:
Human MUC1 (Mucin 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MUC1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MUC1. You can calculate the concentration of Human MUC1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
99-116
88-102
Average (%)
101
106
93
1:4
Range (%)
88-100
86-101
87-103
Average (%)
94
93
95
1:8
Range (%)
95-108
90-102
87-100
Average (%)
101
96
94
1:16
Range (%)
90-105
89-103
96-110
Average (%)
97
95
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
99
EDTA plasma (n=5)
98-111
105
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.57
299.67
967.4
103.18
278.23
1041.74
Standard deviation
10.74
35.03
76.33
9.53
24.15
114.7
C V (%)
11.36
11.69
7.89
9.24
8.68
11.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MUC1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MUC1 in samples. No significant cross-reactivity or interference between Human MUC1 and analogues was observed.
