The Human MT3 (Metallothionein 3) ELISA Kit is specifically designed for the precise measurement of metallothionein 3 levels in human samples such as serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it an indispensable tool for various research purposes.Metallothionein 3 is a key protein involved in the regulation of metal ions and oxidative stress in the body. It plays a critical role in detoxification processes, metal homeostasis, and cellular protection against harmful substances.
Abnormal levels of metallothionein 3 have been linked to various diseases, including cancer, neurodegenerative disorders, and metal toxicity, highlighting its importance as a biomarker for disease progression and treatment efficacy.With the Human MT3 ELISA Kit, researchers can explore the role of metallothionein 3 in health and disease, paving the way for new insights into potential therapeutic interventions and diagnostic strategies. Trust in the accuracy and precision of this kit to advance your research efforts and uncover the mysteries of metallothionein 3 biology.
Product Name:
Human MT3 (Metallothionein 3) ELISA Kit
SKU:
HUES01774
Target:
Human MT3 (Metallothionein 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MT3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MT3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MT3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MT3. You can calculate the concentration of Human MT3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
92-108
91-105
Average (%)
92
99
98
1:4
Range (%)
87-99
82-94
87-99
Average (%)
92
87
92
1:8
Range (%)
88-101
82-96
87-99
Average (%)
95
88
92
1:16
Range (%)
90-104
80-92
85-98
Average (%)
98
86
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
100
EDTA plasma (n=5)
90-104
95
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.45
3.68
0.46
1.59
3.67
Standard deviation
0.02
0.07
0.18
0.02
0.07
0.13
C V (%)
4.35
4.83
4.89
4.35
4.4
3.54
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MT3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MT3 in samples. No significant cross-reactivity or interference between Human MT3 and analogues was observed.
