The Human MT1E (Metallothionein-1E) CLIA Kit is specifically designed for the accurate detection of metallothionein-1E levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing reliable and reproducible results for various research applications.Metallothionein-1E is a crucial protein involved in metal detoxification and homeostasis, playing a significant role in protecting cells from heavy metal toxicity. Dysregulation of metallothionein-1E has been linked to various health conditions such as metal poisoning, neurodegenerative disorders, and cancer, making it a valuable biomarker for understanding these diseases and exploring potential therapeutic interventions.
Overall, the Human MT1E (Metallothionein-1E) CLIA Kit is a valuable tool for researchers studying metal metabolism, toxicology, and related health conditions, offering accurate and reliable results for further advancing scientific understanding in this field.
Product Name:
Human MT1E (Metallothionein 1E) CLIA Kit
SKU:
HUES00399
Target:
Human MT1E (Metallothionein 1E)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MT1E. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MT1E and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MT1E, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MT1E. You can calculate the concentration of Human MT1E in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
97-112
94-109
Average (%)
99
103
99
1:4
Range (%)
95-108
102-119
98-112
Average (%)
102
109
106
1:8
Range (%)
95-108
100-118
88-104
Average (%)
100
108
95
1:16
Range (%)
103-116
84-100
90-105
Average (%)
109
91
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
108
EDTA plasma (n=5)
102-115
108
Cell culture media (n=5)
93-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
219.55
333.99
1664.83
220.29
327.89
1550.56
Standard deviation
23.95
39.85
165.48
25.99
38.43
168.7
C V (%)
10.91
11.93
9.94
11.8
11.72
10.88
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MT1E concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MT1E in samples. No significant cross-reactivity or interference between Human MT1E and analogues was observed.
