The Human MT1 (metallothionein-1) CLIA Kit is specifically designed for the accurate detection of metallothionein-1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Metallothionein-1 is a key protein involved in regulating metal homeostasis and detoxification in the body. Abnormal levels of metallothionein-1 have been associated with various health conditions including heavy metal toxicity, oxidative stress, and cancer.
As such, measuring metallothionein-1 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.Overall, the Human MT1 CLIA Kit is an essential tool for researchers studying metallothionein-1 biology and its implications in human health and disease.
Product Name:
Human MT1 (Metallothionein 1) CLIA Kit
SKU:
HUES00860
Target:
Human MT1 (Metallothionein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MT1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MT1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MT1. You can calculate the concentration of Human MT1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
84-99
102-116
Average (%)
106
91
109
1:4
Range (%)
89-102
96-108
85-98
Average (%)
95
101
91
1:8
Range (%)
91-102
91-104
91-103
Average (%)
96
97
96
1:16
Range (%)
98-110
99-114
91-108
Average (%)
104
107
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
97-109
102
Cell culture media (n=5)
94-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.01
86.61
364.0
38.01
78.16
337.72
Standard deviation
4.17
8.91
34.33
4.8
6.57
36.74
C V (%)
10.97
10.29
9.43
12.63
8.41
10.88
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MT1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MT1 in samples. No significant cross-reactivity or interference between Human MT1 and analogues was observed.
