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Human Microseminoprotein Beta (MSMb) ELISA Kit

SKU:
HUDL01908
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P08118
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MSM-B,IGBF,MSP,MSPB,PN44,PRPS,PSP,PSP57,PSP94,Immunoglobulin-binding factor,Prostate secreted seminal plasma protein,plasma beta-inhibin
Reactivity:
Human
Sample Type:
Serum, Plasma, Seminal Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Microseminoprotein Beta (MSMb) ELISA Kit

The Human Microseminoprotein Beta (MSMB) ELISA Kit is specifically designed for the precise measurement of MSMB levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers dependable and consistent results, making it an invaluable tool for various research purposes.MSMB is a vital protein that plays a key role in prostate health and has been linked to conditions such as prostate cancer.

By accurately measuring MSMB levels, researchers can gain valuable insights into disease progression and potential therapeutic interventions.Overall, the Human MSMB ELISA Kit offers researchers a reliable and efficient method for studying MSMB levels and their implications in various health conditions.

Product Name:Human Microseminoprotein Beta (MSMb) ELISA Kit
Product Code:HUDL01908
Size:96 Assays
Target:MSMb
Synonyms:MSM-B, IGBF, MSP, MSPB, PN44, PRPS, PSP, PSP57, PSP94, Immunoglobulin-binding factor, Prostate secreted seminal plasma protein, plasma beta-inhibin
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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