Human MRPL28 Recombinant Protein (RPPB4003)
- SKU:
- RPPB4003
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q13084
Description
| Product Name: | Human MRPL28 Recombinant Protein |
| Product Code: | RPPB4003 |
| Size: | 10µg |
| Species: | Human |
| Target: | MRPL28 |
| Synonyms: | MAAT1, p15, 39S ribosomal protein L28, mitochondrial, L28mt, MRP-L28, Melanoma-associated antigen recognized by T-lymphocytes. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The MRPL28 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.5), 0.1M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MNGQRERVED VPIPIYFPPE SQRGLWGGEG WILGQIYANN DKLSKRLKKV WKPQLFEREF YSEILDKKFT VTVTMRTLDL IDEAYGLDFY ILKTPKEDLC SKFGMDLKRG MLLRLARQDP QLHPEDPERR AAIYDKYKEF AIPEEEAEWV GLTLEEAIEK QRLLEEKDPV PLFKIYVAEL IQQLQQQALS EPAVVQKRAS GQ |
MRPL28 is found in a variety of normal tissues such as spleen, testis, thymus, liver, kidney, brain, adrenal, lung and retinal tissue. 39S ribosomal protein L28, mitochondrial (MRPL28), is a part of the ribosomal protein L28P family. Mammalian mitochondrial ribosomal proteins encoded by nuclear genes and helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL28 is an important therapeutic reagent for HLA-A24 (A24) patients since this antigen is distinguished by tumor-infiltrating lymphocyte (TIL) 1290, which targets the A24 serotype.
MRPL28 Human Recombinant produced in E. coli is a single polypeptide chain containing 222 amino acids (56-256) and having a molecular mass of 25.8 kDa. MRPL28 is fused to 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MRPL28: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Ribosomal; Mitochondrial; Translation Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: nucleoplasm; mitochondrion; cytoplasm; mitochondrial inner membrane; mitochondrial ribosome Molecular Function:protein binding; structural constituent of ribosome Biological Process: translation; mitochondrial translation; organelle organization and biogenesis |
| NCBI Summary: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13084 |
| NCBI GenInfo Identifier: | 85695426 |
| NCBI Gene ID: | 10573 |
| NCBI Accession: | Q13084.4 |
| UniProt Secondary Accession: | Q13084,Q4TT39, Q96S26, Q9BQD8, Q9BR04, B2RCM4, D3DU46 |
| UniProt Related Accession: | Q13084 |
| Molecular Weight: | 256 |
| NCBI Full Name: | 39S ribosomal protein L28, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial ribosomal protein L28 |
| NCBI Official Symbol: | MRPL28�� |
| NCBI Official Synonym Symbols: | p15; MAAT1�� |
| NCBI Protein Information: | 39S ribosomal protein L28, mitochondrial; L28mt; MRP-L28; melanoma antigen p15; melanoma-associated antigen recognized by T lymphocytes; melanoma-associated antigen recognized by T-lymphocytes; melanoma-associated antigen recognised by cytotoxic T lymphocytes |
| UniProt Protein Name: | 39S ribosomal protein L28, mitochondrial |
| UniProt Synonym Protein Names: | Melanoma antigen p15; Melanoma-associated antigen recognized by T-lymphocytes |
| Protein Family: | 39S ribosomal protein |
| UniProt Gene Name: | MRPL28�� |
| UniProt Entry Name: | RM28_HUMAN |
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