Human MR-ProADM (Mid-regional pro-adrenomedullin) ELISA Kit
The Human MR-ProADM (Mid-Regional Pro-Adrenomedullin) ELISA Kit is specifically designed for the accurate measurement of MR-ProADM levels in human serum samples. This kit offers high sensitivity and specificity, ensuring precise and reliable results for various research applications.MR-ProADM is a biomarker associated with cardiovascular diseases, sepsis, and other inflammatory conditions.
Its measurement can provide valuable insights into disease progression and treatment response, making it a valuable tool for researchers and clinicians alike.With its easy-to-use format and comprehensive instructions, the Human MR-ProADM ELISA Kit from Assay Genie is the perfect choice for studying the role of this important biomarker in various health conditions.
Product Name:
Human MR-ProADM (Mid-regional pro-adrenomedullin) ELISA Kit
SKU:
HUES03440
Target:
Human MR-ProADM (Mid-regional pro-adrenomedullin) ELISA Kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MR-ProADM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MR-ProADM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MR-ProADM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MR-ProADM. You can calculate the concentration of Human MR-ProADM in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
95-109
98-112
Average (%)
97
103
105
1:4
Range (%)
87-99
83-95
86-99
Average (%)
94
88
92
1:8
Range (%)
89-107
83-94
88-99
Average (%)
97
88
94
1:16
Range (%)
86-99
80-94
87-97
Average (%)
93
86
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
84-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.87
137.22
408.52
52.5
125.28
379.42
Standard deviation
3.45
6.28
18.18
3.42
5.76
16.96
C V (%)
6.53
4.58
4.45
6.51
4.6
4.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MR-ProADM concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MR-ProADM in samples. No significant cross-reactivity or interference between Human MR-ProADM and analogues was observed.
