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Human/Mouse MAPK Phosphorylation Array (17 targets) (SARB0055)

SKU:
SARB0055
Product Type:
Phosphorylation Array
Analytes:
Akt (P-Ser473) (PKB (P-Ser473))
CREB (P-Ser133)
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
GSK3a (P-Ser21)
GSK3b (P-Ser9)
HSP27 (P-Ser82)
JNK (P-Thr183)
MEK (P-Ser217/Ser221)
MKK3 (P-Ser189)
MKK6 (P-Ser207)
MSK2 (P-Ser360)
mTOR (P-Ser2448)
p38 (P-Thr180/Tyr182)
P53 (P-Ser15)
P70S6K (P-Thr421/Ser424)
RSK1 (P-Ser380)
RSK2 (P-Ser386)
Reactivity:
Human
Mouse
Applications:
Multiplex Array
€649 - €1,259

Description

system_update_altDatasheet

Human/Mouse MAPK Phosphorylation Array (17 targets) (SARB0055)

The Human/Mouse MAPK Phosphorylation Array (SARB0055) is a powerful tool for studying signal transduction pathways involved in cellular responses to various stimuli. This array allows for the simultaneous detection and quantification of phosphorylation levels of 17 key proteins involved in the MAPK pathway, including MAPKs, MAPK kinases, and MAPK substrates.With high sensitivity and specificity, this array can provide valuable insights into the activation status of MAPK signaling cascades in response to different experimental conditions.

It is suitable for use with both human and mouse samples, making it a versatile tool for researchers studying MAPK signaling in a variety of biological systems.This array is a reliable and cost-effective option for profiling the phosphorylation status of multiple MAPK pathway proteins in a single experiment. It is an essential tool for researchers studying cell signaling pathways, drug discovery, and biomarker identification in the fields of cancer, inflammation, and neurobiology.

Product Sku:SARB0055
Size:2, 4, or 8
Species Detected:Human / Mouse
Number of Targets Detected:17
Gene Symbols:AKT1, CREB1, GSK3A, GSK3B, HSPB1, MAP2K1, MAP2K3, MAP2K6, MAPK1, MAPK14|MAPK8, MTOR, RPS6KA1, RPS6KA3, RPS6KA4, RPS6KB1, TP53
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:Akt Signaling / HER/ErbB Signaling / IGF Signaling / JAK/STAT Signaling / MAPK Signaling / NFkB Signaling / PI3K-AKT Signaling / Wnt/beta-Catenin Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Akt (P-Ser473) (PKB (P-Ser473))
  • Gene Symbol: AKT1 PKB RAC
  • Gene ID: 207
  • Accession #: P31749
CREB (P-Ser133)
  • Gene Symbol: CREB1
  • Gene ID: 1385
  • Accession #: P16220
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
  • Gene Symbol: MAPK1 ERK2 PRKM1 PRKM2
  • Gene ID: 5594
  • Accession #: P28482
GSK3a (P-Ser21)
  • Gene Symbol: GSK3A
  • Gene ID: 2931
  • Accession #: P49840
GSK3b (P-Ser9)
  • Gene Symbol: GSK3B
  • Gene ID: 2932
  • Accession #: P49841
HSP27 (P-Ser82)
  • Gene Symbol: HSPB1 HSP27 HSP28
  • Gene ID: 3315
  • Accession #: P04792
JNK (P-Thr183)
  • Gene Symbol: MAPK8 JNK1 PRKM8 SAPK1 SAPK1C
  • Gene ID: 5599
  • Accession #: P45983
MEK (P-Ser217/Ser221)
  • Gene Symbol: MAP2K1 MEK1 PRKMK1
  • Gene ID: 5604
  • Accession #: Q02750
MKK3 (P-Ser189)
  • Gene Symbol: MAP2K3 MEK3 MKK3 PRKMK3 SKK2
  • Gene ID: 5606
  • Accession #: P46734
MKK6 (P-Ser207)
  • Gene Symbol: MAP2K6 MEK6 MKK6 PRKMK6 SKK3
  • Gene ID: 5608
  • Accession #: P52564
MSK2 (P-Ser360)
  • Gene Symbol: RPS6KA4 MSK2
  • Gene ID: 8986
  • Accession #: O75676
mTOR (P-Ser2448)
  • Gene Symbol: MTOR FRAP FRAP1 FRAP2 RAFT1 RAPT1
  • Gene ID: 2475
  • Accession #: P42345
p38 (P-Thr180/Tyr182)
  • Gene Symbol: MAPK14 CSBP CSBP1 CSBP2 CSPB1 MXI2 SAPK2A
  • Gene ID: 1432
  • Accession #: Q16539
P53 (P-Ser15)
  • Gene Symbol: TP53 P53
  • Gene ID: 7157
  • Accession #: P04637
P70S6K (P-Thr421/Ser424)
  • Gene Symbol: RPS6KB1 STK14A
  • Gene ID: 6198
  • Accession #: P23443
RSK1 (P-Ser380)
  • Gene Symbol: RPS6KA1 MAPKAPK1A RSK1
  • Gene ID: 6195
  • Accession #: Q15418
RSK2 (P-Ser386)
  • Gene Symbol: RPS6KA3 ISPK1 MAPKAPK1B RSK2
  • Gene ID: 10432
  • Accession #: P51812
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human/Mouse MAPK Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit-IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Detection Antibody Cocktail
4.Incubate with HRP-Conjugated anti-IgG
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis

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