Human, Mouse and Rat Phospho-PDK1 (Ser241) PharmaGenie ELISA Kit (SBRS1928)
- SKU:
- SBRS1928
- Product Type:
- ELISA Kit
- Reactivity:
- Human
- Mouse
- Rat
- Applications:
- ELISA
- ELISA Type:
- Sandwich
Frequently bought together:
Description
Human, Mouse and Rat Phospho-PDK1 (Ser241) PharmaGenie ELISA Kit (SBRS1928)
Product SKU: | SBRS1928 |
Size: | 96T |
Application: | This ELISA kit recognizes Human. |
Uniprot: | O15530 / O55173 / Q9Z2A0 |
Gene ID: | 5170 |
Gene Names: | PDK1 / PDPK1 |
Synonyms: | 3-phosphoinositide-dependent protein kinase 1 (hPDK1) (EC 2.7.11.1) |
Target Species: | Human, Mouse, Rat |
Compatible Sample Types: | Cell Lysates, Tissue Lysates |
Design Principle: | Sandwich-based |
Method of Detection: | Colorimetric |
Quantitative/Semi-Quantitative: | Semi-Quantitative |
Storage/Stability: | Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- HRP-Conjugated Secondary Antibody
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
Other materials and equipment required:
The Assay Genie Human, Mouse and Rat Phospho-PDK1 (Ser241) PharmaGenie ELISA Kit (SBRS1928) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 ul to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
- Prepare all reagents and samples as instructed in the manual.
- Add 100 ul of sample or positive control to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 ul of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared 1X HRP-Streptavidin to each well.
- Incubate 1 h at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
HT29 cells were treated with Calyculin A. Cell lysates were treated or untreated with Lambda Protein Phosphatase (LPP) and analyzed using this phosphoELISA and Western Blot. | |
HT29 cells were treated with Calyculin A. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA. |