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Human/Mouse AKT Pathway Phosphorylation Array (18 targets) (SARB0017)

SKU:
SARB0017
Product Type:
Phosphorylation Array
Analytes:
Akt (P-Ser473) (PKB (P-Ser473))
AMPKa (P-Thr172)
BAD (P-Ser112)
4E-BP1 (P-Thr36)
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
GSK3a (P-Ser21)
GSK3b (P-Ser9)
mTOR (P-Ser2448)
p27 (P-Thr198)
P53 (P-Ser15)
P70S6K (P-Thr421/Ser424)
PDK1 (P-Ser241)
PRAS40 (P-Thr246)
PTEN (P-Ser380)
Raf-1 (Ser301)
RPS6 (P-Ser235/Ser236)
RSK1 (P-Ser380)
RSK2 (P-Ser386)
Reactivity:
Human
Mouse
Applications:
Multiplex Array
€559 - €1,089
Frequently bought together:

Description

system_update_altDatasheet

Human/Mouse AKT Pathway Phosphorylation Array (18 targets) (SARB0017)

The Human/Mouse Akt Pathway Phosphorylation Array (SARB0017) is a cutting-edge tool for studying signaling pathways involved in cell growth, survival, and proliferation. This array allows for the simultaneous detection of phosphorylation levels of 18 key proteins in the Akt pathway, providing researchers with valuable insights into cellular signaling mechanisms.With the ability to analyze both human and mouse samples, this array is versatile and suitable for a wide range of experiments. Whether studying cancer biology, cell metabolism, or drug responses, the Human/Mouse Akt Pathway Phosphorylation Array offers a comprehensive view of Akt pathway activation in various biological contexts.

Designed for easy and efficient analysis, this array enables high-throughput screening of multiple phosphorylation events in a single experiment. By identifying changes in protein phosphorylation patterns, researchers can unravel the complex network of signaling cascades that regulate cellular functions. Gain a deeper understanding of Akt pathway activation with the Human/Mouse Akt Pathway Phosphorylation Array and uncover new possibilities for therapeutic interventions in diseases such as cancer and metabolic disorders.

Product Sku:SARB0017
Size:2, 4, or 8
Species Detected:Human / Mouse
Number of Targets Detected:18
Gene Symbols:AKT1, AKT1S1, BAD, CDKN1B, EIF4EBP1, GSK3A, GSK3B, MAPK1, MAPK3, MTOR|PDK1, PRKAA1, PTEN, RAF1, RPS6, RPS6KA1, RPS6KA3, RPS6KB1, TP53
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:Akt Signaling / AMPK Signaling / HER/ErbB Signaling / HIF-1 alpha Signaling / IGF Signaling / JAK/STAT Signaling / MAPK Signaling / mTOR Signaling / PI3K-AKT Signaling / TGF-beta Signaling / Wnt/beta-Catenin Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Akt (P-Ser473) (PKB (P-Ser473))
  • Gene Symbol: AKT1 PKB RAC
  • Gene ID: 207
  • Accession #: P31749
AMPKa (P-Thr172)
  • Gene Symbol: PRKAA1 AMPK1
  • Gene ID: 5562
  • Accession #: Q13131
BAD (P-Ser112)
  • Gene Symbol: BAD BBC6 BCL2L8
  • Gene ID: 572
  • Accession #: Q92934
4E-BP1 (P-Thr36)
  • Gene Symbol: EIF4EBP1
  • Gene ID: 1978
  • Accession #: Q13541
ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187)
  • Gene Symbol: MAPK1 ERK2 PRKM1 PRKM2
  • Gene ID: 5594
  • Accession #: P28482
GSK3a (P-Ser21)
  • Gene Symbol: GSK3A
  • Gene ID: 2931
  • Accession #: P49840
GSK3b (P-Ser9)
  • Gene Symbol: GSK3B
  • Gene ID: 2932
  • Accession #: P49841
mTOR (P-Ser2448)
  • Gene Symbol: MTOR FRAP FRAP1 FRAP2 RAFT1 RAPT1
  • Gene ID: 2475
  • Accession #: P42345
p27 (P-Thr198)
  • Gene Symbol: PSMD9
  • Gene ID: 5715
  • Accession #: O00233
P53 (P-Ser15)
  • Gene Symbol: TP53 P53
  • Gene ID: 6198
  • Accession #: P23443
P70S6K (P-Thr421/Ser424)
  • Gene Symbol: RPS6KB1 STK14A
  • Gene ID: 6198
  • Accession #: P23443
PDK1 (P-Ser241)
  • Gene Symbol: PDK1 PDHK1
  • Gene ID: 5170
  • Accession #: O15530
PRAS40 (P-Thr246)
  • Gene Symbol: AKT1S1 PRAS40
  • Gene ID: 84335
  • Accession #: Q96B36
PTEN (P-Ser380)
  • Gene Symbol: N/A
  • Gene ID: N/A
  • Accession #: P60484; O08586; O54857
Raf-1 (Ser301)
  • Gene Symbol: RAF1 RAF
  • Gene ID: 5894
  • Accession #: P04049
RPS6 (P-Ser235/Ser236)
  • Gene Symbol: RPS6 OK/SW-cl.2
  • Gene ID: 6194
  • Accession #: P62753
RSK1 (P-Ser380)
  • Gene Symbol: RPS6KA1 MAPKAPK1A RSK1
  • Gene ID: 6195
  • Accession #: Q15418
RSK2 (P-Ser386)
  • Gene Symbol: RPS6KA3 ISPK1 MAPKAPK1B RSK2
  • Gene ID: 10432
  • Accession #: P51812
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human and Mouse AKT Pathway Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Detection Antibody Cocktail
4.Incubate with HRP-Conjugated anti-IgG
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis

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