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Human Monokine Induced By Interferon Gamma (MIg) ELISA Kit

SKU:
HUDL01854
Product Type:
ELISA Kit
Sensitivity:
0.062ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CXCL9,CMK,Humig,MI-G,SCYB9,Crg-10,Chemokine (C-X-C motif) ligand 9,Small-inducible cytokine B9,Gamma-interferon-induced monokine
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$719
Frequently bought together:

Description

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Human Monokine Induced By Interferon Gamma (MIg) ELISA Kit

The Human Monokine Induced by Interferon gamma (MIG) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of MIG levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.MIG, also known as CXCL9, is a chemokine that is induced by interferon gamma and plays a key role in immune responses, inflammation, and the recruitment of immune cells to sites of infection or tissue damage.

Elevated levels of MIG have been associated with various autoimmune and inflammatory disorders, making it an important biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human MIG ELISA Kit is a valuable tool for researchers studying immune responses, inflammation, and autoimmune diseases, providing accurate quantification of MIG levels in human samples.

Product Name:Human Monokine Induced By Interferon Gamma (MIg) ELISA Kit
Product Code:HUDL01854
Size:96 Assays
Target:MIg
Synonyms:CXCL9, CMK, Humig, MI-G, SCYB9, Crg-10, Chemokine (C-X-C motif) ligand 9, Small-inducible cytokine B9, Gamma-interferon-induced monokine
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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