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Human Monocyte Chemotactic Protein 4 (MCP4) ELISA Kit

SKU:
HUDL01817
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q99616
Sensitivity:
7.8pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CCL13,SCYA13,NCC1,SCYL1,CKb10,Chemokine C-C-Motif Ligand 13,Small Inducible Cytokine Subfamily A(Cys-Cys)Member 13,CK-beta-10,Monocyte chemoattractant 4
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Monocyte Chemotactic Protein 4 (MCP4) ELISA Kit

The Human Monocyte Chemotactic Protein 4 (MCP4) ELISA Kit is a reliable tool for detecting MCP4 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.MCP4 is a critical chemokine involved in the recruitment and activation of monocytes, playing a key role in immune responses and inflammation. Dysregulation of MCP4 levels has been implicated in various inflammatory conditions, making it an important biomarker for studying and potentially treating these diseases.

With its high-performance capabilities, the Human MCP4 ELISA Kit is the ideal choice for researchers looking to investigate the role of MCP4 in various disease processes and explore potential therapeutic targets. Get reliable data and meaningful insights with this advanced ELISA kit.

Product Name:Human Monocyte Chemotactic Protein 4 (MCP4) ELISA Kit
Product Code:HUDL01817
Size:96 Assays
Target:MCP4
Synonyms:CCL13, SCYA13, NCC1, SCYL1, CKb10, Chemokine C-C-Motif Ligand 13, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 13, CK-beta-10, Monocyte chemoattractant 4
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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