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Human Monoacylglycerol-O-acyltransferase 2 (MOGAT2) ELISA Kit

SKU:
HUDL01890
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q3SYC2
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MGAT2,DGAT2L5,DC5,2-Acylglycerol O-Acyltransferase 2,Diacylglycerol O-acyltransferase candidate 5,Diacylglycerol acyltransferase 2-like protein 5
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Monoacylglycerol-O-acyltransferase 2 (MOGAT2) ELISA Kit

The Human Monoacylglycerol O-acyltransferase 2 (MOGAT2) ELISA Kit is specifically designed for the accurate and reliable detection of MOGAT2 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.MOGAT2 is an important enzyme involved in lipid metabolism, specifically in the synthesis of triglycerides. Dysregulation of MOGAT2 has been associated with metabolic disorders such as obesity, diabetes, and cardiovascular disease, making it a valuable target for studying these conditions and developing potential treatments.

With the Human MOGAT2 ELISA Kit, researchers can confidently measure MOGAT2 levels in biological samples, enabling deeper insights into lipid metabolism and its role in metabolic diseases. Get accurate results and advance your research with this innovative ELISA kit from AssayGenie.

Product Name:Human Monoacylglycerol-O-acyltransferase 2 (MOGAT2) ELISA Kit
Product Code:HUDL01890
Size:96 Assays
Target:MOGAT2
Synonyms:MGAT2, DGAT2L5, DC5, 2-Acylglycerol O-Acyltransferase 2, Diacylglycerol O-acyltransferase candidate 5, Diacylglycerol acyltransferase 2-like protein 5
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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