The Human MMRN1 (Multimerin-1) ELISA Kit is specially designed to accurately measure levels of Multimerin-1 in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring dependable and consistent results for various research purposes.Multimerin-1 is a key protein involved in various biological processes, including blood clotting, wound healing, and vascular development.
Its dysregulation has been linked to conditions such as thrombosis, inflammation, and cardiovascular diseases, making it a valuable biomarker for understanding these pathologies and exploring potential therapeutic interventions.With its easy-to-use format and reliable performance, the Human MMRN1 ELISA Kit is an essential tool for researchers studying Multimerin-1 biology and its implications in human health and disease.
Product Name:
Human MMRN1 (Multimerin 1) ELISA Kit
SKU:
HUES01754
Target:
Human MMRN1 (Multimerin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMRN1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMRN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMRN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMRN1. You can calculate the concentration of Human MMRN1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
92-108
94-105
Average (%)
101
99
100
1:4
Range (%)
94-105
86-97
81-95
Average (%)
100
92
88
1:8
Range (%)
93-108
82-93
81-96
Average (%)
100
87
88
1:16
Range (%)
90-103
82-97
82-94
Average (%)
96
89
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
91
EDTA plasma (n=5)
94-109
100
Cell culture media (n=5)
89-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
236.7
545.51
1682.79
222.73
492.13
1789.76
Standard deviation
16.31
31.31
83.3
11.96
22.83
69.98
C V (%)
6.89
5.74
4.95
5.37
4.64
3.91
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MMRN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMRN1 in samples. No significant cross-reactivity or interference between Human MMRN1 and analogues was observed.
