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Human MMP14 Recombinant Protein (RPPB1973)

SKU:
RPPB1973
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P50281
Research Area:
Enzymes
$1,523
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Description

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Product Name:Human MMP14 Recombinant Protein
Product Code:RPPB1973
Size:100µg
Species:Human
Target:MMP14
Synonyms:Matrix Metallopeptidase 14, Matrix Metallopeptidase 14 (Membrane-Inserted), Membrane-Type-1 Matrix Metalloproteinase, Membrane Type 1 Metalloprotease, EC 3.4.24.80, MT-MMP 1, MT1-MMP, MMP-14, MMP-X1, MT1MMP, MTMMP1, Matrix Metalloproteinase 14 (Membrane-Inserted), Membrane-Type Matrix Metalloproteinase 1, Matrix Metalloproteinase-14, EC 3.4.24, MT-MMP, WNCHRS, Matrix metalloproteinase-14, Membrane-type matrix metalloproteinase 1.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:MMP14 is supplied as a 0.2 ?m filtered solution conteining 20mM Tris-HCl, pH 7.4, 30 % glycerol, 300mM NaCl, 3mM CaCl2 and 10?M ZnCl2.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:ALASLGSAQS SSFSPEAWLQ QYGYLPPGDL RTHTQRSPQS LSAAIAAMQK FYGLQVTGKA DADTMKAMRR PRCGVPDKFG AEIKANVRRK RYAIQGLKWQ HNEITFCIQN YTPKVGEYAT YEAIRKAFRV WESATPLRFR EVPYAYIREG HEKQADIMIF FAEGFHGDST PFDGEGGFLA HAYFPGPNIG GDTHFDSAEP WTVRNEDLNG NDIFLVAVHE LGHALGLEHS SDPSAIMAPF YQWMDTENFV LPDDDRRGIQ QLYG

Matrix metalloproteinase-14 (MMP14), is a membrane-anchored zinc-binding endopeptidase which is expressed at the leading edge of different invasive carcinomas and also promotes tumor cell invasion through degradation of the extracellular matrix.� MMP14 takes a vital part in extracellular matrix, ECM, remodeling by having the capability to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules for instance CD44 and integrin alpha V. MMP14 is a key enzyme in many physiological as well as pathological processes for example angiogenesis & tumor invasion.

Matrix Metalloproteinase-14 Human Recombinant produced in E.Coli is a single, non- glycosylated polypeptide chain containing 264 amino acids and having a molecular mass of 29.6kDa.MMP14 is purified by proprietary chromatographic techniques.

UniProt Protein Function:MMP14: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Up-regulated by NANOS1. Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. Belongs to the peptidase M10A family.
UniProt Protein Details:

Protein type:EC 3.4.24.80; Protease; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular matrix; focal adhesion; Golgi lumen; integral to plasma membrane; cytoplasm; melanosome; plasma membrane

Molecular Function:integrin binding; protein binding; protease activator activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding; transcription factor activity

Biological Process: extracellular matrix organization and biogenesis; tissue remodeling; negative regulation of focal adhesion formation; male gonad development; embryonic cranial skeleton morphogenesis; proteolysis; positive regulation of cell growth; collagen catabolic process; extracellular matrix disassembly; regulation of transcription, DNA-dependent; branching morphogenesis of a tube; ovarian follicle development; response to estrogen stimulus; response to mechanical stimulus; endothelial cell proliferation; response to hypoxia; zymogen activation; response to oxidative stress; angiogenesis; astrocyte cell migration; lung development; positive regulation of cell migration; endochondral ossification

Disease: Winchester Syndrome

NCBI Summary:Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]
UniProt Code:P50281
NCBI GenInfo Identifier:317373419
NCBI Gene ID:4323
NCBI Accession:P50281.3
UniProt Secondary Accession:P50281,Q6GSF3, Q92678, A8K5L0,
UniProt Related Accession:P50281
Molecular Weight:65,894 Da
NCBI Full Name:Matrix metalloproteinase-14
NCBI Synonym Full Names:matrix metallopeptidase 14 (membrane-inserted)
NCBI Official Symbol:MMP14��
NCBI Official Synonym Symbols:MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1��
NCBI Protein Information:matrix metalloproteinase-14; membrane type 1 metalloprotease; membrane-type-1 matrix metalloproteinase
UniProt Protein Name:Matrix metalloproteinase-14
UniProt Synonym Protein Names:MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP
Protein Family:Matrix metalloproteinase
UniProt Gene Name:MMP14��
UniProt Entry Name:MMP14_HUMAN

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