Human MMP 8 Recombinant Protein (RPPB1963)
- SKU:
- RPPB1963
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P22894
- Research Area:
- Enzymes
Description
| Product Name: | Human MMP 8 Recombinant Protein |
| Product Code: | RPPB1963 |
| Size: | 20µg |
| Species: | Human |
| Target: | MMP 8 |
| Synonyms: | CLG1, HNC, MMP-8, PMNL-CL, Neutrophil collagenase, Matrix metalloproteinase-8, MMP-8, PMNL collagenase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The MMP 8 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSLTPGNPK WERTNLTYRI RNYTPQLSEA EVERAIKDAF ELWSVASPLI FTRISQGEAD INIAFYQRDH GDNSPFDGPN GILAHAFQPG QGIGGDAHFD AEETWTNTSA NYNLFLVAAH EFGHSLGLAH SSDPGALMYP NYAFRETSNY SLPQDDIDGI QAIYGLSSNP IQPTGPSTPK PCDPSLTFDA ITTLRGEILF FKDRYFWRRH PQLQRVEMNF ISLFWPSLPT GIQAAYEDFD RDLIFLFKGN QYWALSGYDI LQGYPKDISN YGFPSSVQAI DAAVFYRSKT YFFVNDQFWR YDNQRQFMEP GYPKSISGAF PGIESKVDAV FQQEHFFHVF SGPRYYAFDL IAQRVTRVAR GNKWLNCRYG |
Full-length recombinant human neutrophil pro-collagenase (MMP-8), latent form.Matrix metalloproteinase 8 (MMP-8), or neutrophil collagenase, degrades interstitial collagens, acting preferentially on collagen type I. Increased full-length MMP-8 protein was associated with infiltration into the skin of neutrophils, which are the major cell type that expresses MMP-8.MMP-8 is synthesized and stored in specific granules in neutrophil leukocytes. MMP-8 activity is therefore regulated by factors such as surface-bound ligands (IgG or complement components) that release it through degranulation.Once released and activated through proteolytic or oxidative mechanisms, MMP-8 plays a major role in the connective tissue turnover that accompanies inflammatory processes.
MMP 8 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 390 amino acids (101-467a.a) and having a molecular mass of 44.3kDa. MMP 8 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family. |
| UniProt Protein Details: | Protein type:Protease; EC 3.4.24.34; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 11q22.3 Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region Molecular Function:zinc ion binding; serine-type endopeptidase activity; metalloendopeptidase activity; calcium ion binding Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; proteolysis |
| NCBI Summary: | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P22894 |
| NCBI GenInfo Identifier: | 116862 |
| NCBI Gene ID: | 4317 |
| NCBI Accession: | P22894.1 |
| UniProt Secondary Accession: | P22894,Q45F99, |
| UniProt Related Accession: | P22894 |
| Molecular Weight: | 53,412 Da |
| NCBI Full Name: | Neutrophil collagenase |
| NCBI Synonym Full Names: | matrix metallopeptidase 8 (neutrophil collagenase) |
| NCBI Official Symbol: | MMP8�� |
| NCBI Official Synonym Symbols: | HNC; CLG1; MMP-8; PMNL-CL�� |
| NCBI Protein Information: | neutrophil collagenase; PMNL collagenase; matrix metalloproteinase-8; matrix metalloproteinase 8 (neutrophil collagenase) |
| UniProt Protein Name: | Neutrophil collagenase |
| UniProt Synonym Protein Names: | Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL |
| Protein Family: | Neutrophil collagenase |
| UniProt Gene Name: | MMP8�� |
| UniProt Entry Name: | MMP8_HUMAN |
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