Human MMP 7 Recombinant Protein (RPPB1962)
- SKU:
- RPPB1962
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P09237
- Research Area:
- Enzymes
Description
| Product Name: | Human MMP 7 Recombinant Protein |
| Product Code: | RPPB1962 |
| Size: | 20µg |
| Species: | Human |
| Target: | MMP 7 |
| Synonyms: | Matrilysin, EC 3.4.24.23, Pump-1 protease, Uterine metalloproteinase, Matrix metalloproteinase-7, MMP-7, Matrin, MPSL1, PUMP-1, MMP7. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | MMP7 protein solution (1mg/ml) containing 20mM Tris 8.0 and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MYSLFPNSPK WTSKVVTYRI VSYTRDLPHI TVDRLVSKAL NMWGKEIPLH FRKVVWGTAD IMIGFARGAH GDSYPFDGPG NTLAHAFAPG TGLGGDAHFD EDERWTDGSS LGINFLYAAT HELGHSLGMG HSSDPNAVMY PTYGNGDPQN FKLSQDDIKG IQKLYGKRSN SRKK |
Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28 kDa proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18 kDa active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of gliomas.
MMP-7 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 174 amino acids (95-267 a.a) and having a molecular mass of 19.2kDa.MMP7 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MMP7: Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. Belongs to the peptidase M10A family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide; Protease; EC 3.4.24.23 Chromosomal Location of Human Ortholog: 11q22.2 Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region Molecular Function:zinc ion binding; metalloendopeptidase activity Biological Process: response to drug; collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; defense response to Gram-positive bacterium; antibacterial peptide biosynthetic process; defense response to Gram-negative bacterium; proteolysis; antibacterial peptide secretion; regulation of cell proliferation |
| NCBI Summary: | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P09237 |
| NCBI GenInfo Identifier: | 116861 |
| NCBI Gene ID: | 4316 |
| NCBI Accession: | P09237.1 |
| UniProt Secondary Accession: | P09237,Q9BTK9, |
| UniProt Related Accession: | P09237 |
| Molecular Weight: | 29,677 Da |
| NCBI Full Name: | Matrilysin |
| NCBI Synonym Full Names: | matrix metallopeptidase 7 (matrilysin, uterine) |
| NCBI Official Symbol: | MMP7�� |
| NCBI Official Synonym Symbols: | MMP-7; MPSL1; PUMP-1�� |
| NCBI Protein Information: | matrilysin; matrin; pump-1 protease; uterine matrilysin; uterine metalloproteinase; matrix metalloproteinase-7; matrix metalloproteinase 7 (matrilysin, uterine) |
| UniProt Protein Name: | Matrilysin |
| UniProt Synonym Protein Names: | Matrin; Matrix metalloproteinase-7; MMP-7; Pump-1 protease; Uterine metalloproteinase |
| Protein Family: | Matrilysin |
| UniProt Gene Name: | MMP7�� |
| UniProt Entry Name: | MMP7_HUMAN |
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