Human MMP-7 (Matrix Metalloproteinase 7) ELISA Kit
The Human MMP-7 (Matrix Metalloproteinase 7) ELISA Kit is a reliable and accurate tool for measuring MMP-7 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and consistent results for various research applications. MMP-7, also known as matrilysin, is a key enzyme involved in the breakdown of extracellular matrix proteins, playing a crucial role in tissue remodeling and cell migration. Elevated levels of MMP-7 have been associated with various diseases including cancer, inflammatory conditions, and cardiovascular disorders, making it a valuable biomarker for studying disease progression and potential therapeutic interventions.
With the Human MMP-7 ELISA Kit, researchers can accurately measure MMP-7 levels in biological samples, providing valuable insights into disease mechanisms and potential treatment strategies. This kit is a valuable tool for researchers working in the fields of cancer biology, inflammation, and tissue remodeling.
Product Name:
Human MMP-7 (Matrix Metalloproteinase 7) ELISA Kit
SKU:
HUES02484
Target:
Human MMP-7 (Matrix Metalloproteinase 7)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-7. You can calculate the concentration of Human MMP-7 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
94-107
87-101
Average (%)
102
99
93
1:4
Range (%)
96-112
83-96
93-105
Average (%)
102
90
98
1:8
Range (%)
96-107
83-94
95-107
Average (%)
101
88
101
1:16
Range (%)
99-113
84-95
93-109
Average (%)
106
89
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
95
EDTA plasma (n=5)
96-112
102
Cell culture media (n=5)
90-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
0.9
4.9
0.5
1.0
5.2
Standard deviation
0.03
0.04
0.17
0.03
0.05
0.18
C V (%)
6
4.44
3.47
6.0
5.0
3.46
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MMP-7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-7 in samples. No significant cross-reactivity or interference between Human MMP-7 and analogues was observed.
