Human MMP-3 (Matrix Metalloproteinase 3) ELISA Kit
The Human MMP-3 (Matrix Metalloproteinase 3) ELISA Kit is designed for the precise measurement of MMP-3 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results, making it an indispensable tool for a variety of research applications.MMP-3, also known as stromelysin-1, is a key enzyme involved in tissue remodeling and extracellular matrix degradation. Its dysregulation has been linked to various pathological conditions including arthritis, cardiovascular diseases, and cancer.
By measuring MMP-3 levels, researchers can gain valuable insights into disease progression and potential therapeutic targets.Overall, the Human MMP-3 ELISA Kit provides researchers with a reliable and efficient way to study the role of MMP-3 in various diseases, ultimately advancing our understanding of disease mechanisms and aiding in the development of novel treatment strategies.
Product Name:
Human MMP-3 (Matrix Metalloproteinase 3) ELISA Kit
SKU:
HUES02481
Target:
Human MMP-3 (Matrix Metalloproteinase 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-3. You can calculate the concentration of Human MMP-3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
86-98
95-108
Average (%)
94
92
101
1:4
Range (%)
93-108
86-100
97-109
Average (%)
101
91
102
1:8
Range (%)
93-109
81-95
95-107
Average (%)
101
87
100
1:16
Range (%)
97-112
85-101
96-109
Average (%)
105
92
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
96
EDTA plasma (n=5)
94-106
101
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.3
4.5
0.5
1.4
4.8
Standard deviation
0.03
0.07
0.24
0.03
0.07
0.14
C V (%)
6
5.38
5.33
6.0
5.0
2.92
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MMP-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-3 in samples. No significant cross-reactivity or interference between Human MMP-3 and analogues was observed.
