Human MMP-2 (Matrix Metalloproteinase 2) ELISA Kit
The Human MMP-2 (Matrix Metalloproteinase 2) ELISA Kit is a reliable tool for the precise measurement of MMP-2 levels in human biological samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, providing accurate and reproducible results, making it ideal for a wide range of research applications.MMP-2, also known as gelatinase A, is a key enzyme involved in the breakdown of extracellular matrix proteins, playing a critical role in processes such as tissue remodeling, wound healing, and tumor metastasis.
Abnormal MMP-2 levels have been linked to various diseases including cancer, cardiovascular conditions, and inflammatory disorders, making it a valuable biomarker for studying these pathological conditions and exploring potential therapeutic strategies.Overall, the Human MMP-2 ELISA Kit offers researchers a valuable tool for studying the role of MMP-2 in disease pathogenesis and for developing targeted interventions to modulate its activity.
Product Name:
Human MMP-2 (Matrix Metalloproteinase 2) ELISA Kit
SKU:
HUES02480
Target:
Human MMP-2 (Matrix Metalloproteinase 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-2. You can calculate the concentration of Human MMP-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-112
94-110
90-104
Average (%)
105
102
96
1:4
Range (%)
97-114
87-101
94-108
Average (%)
105
92
101
1:8
Range (%)
96-110
80-94
97-111
Average (%)
102
87
103
1:16
Range (%)
101-116
83-95
95-110
Average (%)
107
88
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
87-99
92
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.4
5.3
21.8
2.4
5.1
20.8
Standard deviation
0.1
0.3
0.9
0.2
0.2
0.7
C V (%)
4.17
5.66
4.13
8.33
3.92
3.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MMP-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP-2 in samples. No significant cross-reactivity or interference between Human MMP-2 and analogues was observed.
