Human MMP 13 Recombinant Protein (RPPB1954)
- SKU:
- RPPB1954
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P45452
- Research Area:
- Enzymes
Description
| Product Name: | Human MMP 13 Recombinant Protein |
| Product Code: | RPPB1954 |
| Size: | 10µg |
| Species: | Human |
| Target: | MMP 13 |
| Synonyms: | CLG3, MANDP1, Matrix metalloproteinase-13, MMP-13, MMP13. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The MMP-13 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.15M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSYNVFPRT LKWSKMNLTY RIVNYTPDMT HSEVEKAFKK AFKVWSDVTP LNFTRLHDGI ADIMISFGIK EHGDFYPFDG PSGLLAHAFP PGPNYGGDAH FDDDETWTSS SKGYNLFLVA AHEFGHSLGL DHSKDPGALM FPIYTYTGKS HFMLPDDDVQ GIQSLYGPGD EDPNPKHPKT PDKCDPSLSL DAITSLRGET MIFKDRFFWR LHPQQVDAEL FLTKSFWPEL PNRIDAAYEH PSHDLIFIFR GRKFWALNGY DILEGYPKKI SELGLPKEVK KISAAVHFED TGKTLLFSGN QVWRYDDTNH IMDKDYPRLI EEDFPGIGDK VDAVYEKNGY IYFFNGPIQF EYSIWSNRIV RVMPANSILW C |
Matrix Metalloproteinase-13 (MMP-13) is an enzyme that is a member of the MMP extracellular protease family. Extracellular protease enzymes, by virtue of their broad substrate specificities1, play a role in both normal and disease states of tissue proliferation. Among the targets of MMP-13 are collagen, gelatin, entactin, pro-TNF-a, and chemokine SDF-11-4.MMP-13 is found in its latent form as a 52-56 kDa glycosylated proenzyme. Upon cleavage the 22-46 kDa5 MMP-1 becomes active in extracellular matrix remodeling.Because of the prominent role that MMP-1 plays in cell migration and metastasis, it is an important target for inhibition screening.
MMP-13 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 391 amino acids (104-471 a.a.) and having a molecular mass of 44.7 kDa. MMP-13 is fused to a 23 amino acid His Tag at N-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MMP13: Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO). A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age. Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1). Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. Belongs to the peptidase M10A family. |
| UniProt Protein Details: | Protein type:Secreted; EC 3.4.24.-; Secreted, signal peptide; Protease Chromosomal Location of Human Ortholog: 11q22.3 Cellular Component: extracellular region Molecular Function:calcium ion binding; collagen binding; metalloendopeptidase activity; serine-type endopeptidase activity; zinc ion binding Biological Process: collagen catabolic process; extracellular matrix disassembly Disease: Spondyloepimetaphyseal Dysplasia, Missouri Type |
| NCBI Summary: | This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016] |
| UniProt Code: | P45452 |
| NCBI GenInfo Identifier: | 1168998 |
| NCBI Gene ID: | 4322 |
| NCBI Accession: | P45452.1 |
| UniProt Secondary Accession: | P45452,Q6NWN6, A8K846, B2RCZ3, |
| UniProt Related Accession: | P45452 |
| Molecular Weight: | 53,820 Da |
| NCBI Full Name: | Collagenase 3 |
| NCBI Synonym Full Names: | matrix metallopeptidase 13 |
| NCBI Official Symbol: | MMP13�� |
| NCBI Official Synonym Symbols: | CLG3; MANDP1; MMP-13�� |
| NCBI Protein Information: | collagenase 3 |
| UniProt Protein Name: | Collagenase 3 |
| UniProt Synonym Protein Names: | Matrix metalloproteinase-13; MMP-13 |
| Protein Family: | Collagenase |
| UniProt Gene Name: | MMP13�� |
| UniProt Entry Name: | MMP13_HUMAN |
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