Human MMP 1 Recombinant Protein (RPPB1950)
- SKU:
- RPPB1950
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P03956
- Research Area:
- Enzymes
Description
| Product Name: | Human MMP 1 Recombinant Protein |
| Product Code: | RPPB1950 |
| Size: | 10µg |
| Species: | Human |
| Target: | MMP 1 |
| Synonyms: | Interstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1, MMP1, CLG, CLGN. |
| Source: | HEK293 Cells |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The MMP-1 is supplied as a 0.2�m filtered solution in MES, NaCl, Glycerol and Brij35. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Biological Activity: | The activity was measured by its ability to cleave fluorogenic peptide substrate, Mca-KPLGL-Dpa-AR-NH2, The specific activity is > 400 pmoles/min/�g.Recombinant Human MMP-1 protein pro form needs to be activated with p-aminophenylmercuric acetate (APMA).Activation Protocol:1. Dilute MMP1 to 50�g/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP1 by adding APMA to a final concentration of 1mM. (Sigma, Catalog # A9563) and 100mM stock in DMSO.3. Incubate at 37�C for 2 hours. |
MMP-1 (interstitial collagenase) can break down a wide range of substrates including types I, II, III, VII, VIII, and X collagens as well as L-Selectin, pro-TNF, IL-1b, IGFBP-3, IGFBP-5, casein, gelatin, a1 antitrypsin, myelin basic protein, pro-MMP2 and pro-MMP9. A significant function of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling. MMP-1 is expressed in fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP1 can be divided into a number of distinct domains: a prodomain which is cleaved on activation, a catalytic domain containing the zinc binding site and a short hinge region with a carboxyl terminal domain. MMP1 is part of a cluster of MMP genes which localize to chromosome 11q22.3.
MMP-1 Human Recombinant produced in HEK293 cells is a proform of the Human MMP1 (Met1-Asn469) and fused with a ployhistide tag at the C-terminus, having an Mw of 52kDa. MMP-1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MMP1: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Belongs to the peptidase M10A family. |
| UniProt Protein Details: | Protein type:EC 3.4.24.7; Protease; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide Chromosomal Location of Human Ortholog: 11q22.3 Cellular Component: extracellular region Molecular Function:endopeptidase activity; metalloendopeptidase activity; serine-type endopeptidase activity Biological Process: cellular protein metabolic process; collagen catabolic process; extracellular matrix disassembly; leukocyte migration; positive regulation of protein oligomerization; proteolysis Disease: Epidermolysis Bullosa Dystrophica, Autosomal Recessive; Pulmonary Disease, Chronic Obstructive |
| NCBI Summary: | This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016] |
| UniProt Code: | P03956 |
| NCBI GenInfo Identifier: | 116852 |
| NCBI Gene ID: | 4312 |
| NCBI Accession: | P03956.3 |
| UniProt Secondary Accession: | P03956,P08156, |
| UniProt Related Accession: | P03956 |
| Molecular Weight: | 54,007 Da |
| NCBI Full Name: | Interstitial collagenase |
| NCBI Synonym Full Names: | matrix metallopeptidase 1 |
| NCBI Official Symbol: | MMP1�� |
| NCBI Official Synonym Symbols: | CLG; CLGN�� |
| NCBI Protein Information: | interstitial collagenase |
| UniProt Protein Name: | Interstitial collagenase |
| UniProt Synonym Protein Names: | Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1 |
| Protein Family: | Interstitial collagenase |
| UniProt Gene Name: | MMP1�� |
| UniProt Entry Name: | MMP1_HUMAN |
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