The Human MMP-1 (Matrix Metalloproteinase-1) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of MMP-1 levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit delivers reliable and reproducible results, making it an invaluable tool for researchers studying the role of MMP-1 in various conditions.Matrix Metalloproteinase-1 is a key enzyme involved in extracellular matrix degradation and tissue remodeling. It plays a crucial role in processes such as wound healing, tissue repair, and inflammation.
Abnormal MMP-1 levels have been associated with diseases such as arthritis, cancer, and cardiovascular disorders, highlighting its importance as a biomarker for disease progression and therapeutic targeting.With its high-performance capabilities, the Human MMP-1 CLIA Kit offers researchers a powerful means to study MMP-1 expression and activity in a variety of biological systems. Its user-friendly format and reliable results make it an essential tool for investigating the role of MMP-1 in health and disease.
Product Name:
Human MMP-1 (Matrix Metalloproteinase 1) CLIA Kit
SKU:
HUES00818
Target:
Human MMP-1 (Matrix Metalloproteinase 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MMP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MMP1. You can calculate the concentration of Human MMP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
89-100
97-113
Average (%)
95
94
104
1:4
Range (%)
93-103
101-117
96-110
Average (%)
98
107
102
1:8
Range (%)
91-104
84-99
97-113
Average (%)
97
90
104
1:16
Range (%)
96-109
103-116
90-106
Average (%)
102
109
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
100
EDTA plasma (n=5)
95-109
103
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
95.52
319.84
707.08
96.4
334.06
672.4
Standard deviation
9.83
33.58
73.96
11.77
24.22
43.77
C V (%)
10.29
10.5
10.46
12.21
7.25
6.51
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MMP1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MMP1 in samples. No significant cross-reactivity or interference between Human MMP1 and analogues was observed.
