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Human Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) ELISA Kit

SKU:
HUDL01789
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q02750
Sensitivity:
0.056ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MAPKK1,MEK1,MKK1,PRKMK1,Dual specificity mitogen-activated protein kinase kinase 1,ERK activator kinase 1
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) ELISA Kit

The Human Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) ELISA Kit is a highly specific and sensitive assay designed for the quantitative measurement of MAP2K1 levels in human serum, plasma, and cell culture supernatants. MAP2K1, also known as MEK1, is a key mediator in the MAPK signaling pathway, playing a critical role in cell growth, differentiation, and apoptosis.This ELISA kit provides reliable and reproducible results, allowing researchers to accurately assess MAP2K1 levels in various biological samples.

By studying MAP2K1 expression, scientists can gain valuable insights into cell signaling pathways, cancer development, and potential therapeutic targets.Overall, the Human MAP2K1 ELISA Kit is an essential tool for studying cellular signaling pathways and identifying potential targets for therapeutic intervention in diseases such as cancer and other MAPK pathway-related disorders.

Product Name:Human Mitogen Activated Protein Kinase Kinase 1 (MAP2K1) ELISA Kit
Product Code:HUDL01789
Size:96 Assays
Target:MAP2K1
Synonyms:MAPKK1, MEK1, MKK1, PRKMK1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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