The Human Mitoferrin (MFRN) ELISA Kit is a powerful tool for the precise measurement of mitoferrin levels in human biological samples such as serum, plasma, and cell culture supernatants. With its outstanding sensitivity and specificity, this kit delivers accurate and reproducible results, making it well-suited for a wide array of research purposes.Mitoferrin is a key protein involved in iron transport within mitochondria, essential for cellular energy production and metabolism. Dysregulation of mitoferrin has been linked to various diseases, including iron metabolism disorders and mitochondrial dysfunction.
By detecting mitoferrin levels, researchers can gain valuable insights into these conditions and potentially identify new therapeutic targets.The Human Mitoferrin (MFRN) ELISA Kit is a valuable tool for researchers studying iron homeostasis, mitochondrial function, and related diseases. Its high performance and reliability make it a crucial addition to any research laboratory's toolkit.
Product Name:
Human Mitoferrin (MFRN) ELISA Kit
Product Code:
HUDL01838
Size:
96 Assays
Target:
MFRN
Synonyms:
SLC25A37, MSC, HT015, MSCP, Solute Carrier Family 25 Member 37, Mitochondrial iron transporter 1, Mitochondrial solute carrier protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
