Human MIP-3 beta/ELC/CCL19 (Macrophage Inflammatory Protein 3 beta) CLIA Kit
The Human MIP-3 Beta ELC (CCL19) CLIA Kit is designed for the accurate detection of macrophage inflammatory protein-3 beta levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.Macrophage inflammatory protein-3 beta, also known as CCL19, is a chemokine involved in the recruitment and activation of immune cells, particularly dendritic cells and T cells.
It plays a crucial role in inflammation, immune responses, and the migration of immune cells to specific tissues.This CLIA kit provides researchers with a valuable tool for studying the role of MIP-3 beta in various diseases, including inflammatory disorders, autoimmune diseases, and cancer. By accurately measuring MIP-3 beta levels, researchers can gain insights into the underlying mechanisms of these conditions and potentially develop new therapeutic interventions.
Product Name:
Human MIP-3 beta/ELC/CCL19 (Macrophage Inflammatory Protein 3 beta) CLIA Kit
SKU:
HUES00866
Target:
Human MIP-3 beta/ELC/CCL19 (Macrophage Inflammatory Protein 3 beta)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MIP-3β/ELC/CCL19. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIP-3β/ELC/CCL19 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIP-3β/ELC/CCL19, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MIP-3β/ELC/CCL19. You can calculate the concentration of Human MIP-3β/ELC/CCL19 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-103
90-102
100-113
Average (%)
98
96
106
1:4
Range (%)
91-103
84-98
88-101
Average (%)
97
91
95
1:8
Range (%)
92-106
94-105
99-112
Average (%)
98
99
105
1:16
Range (%)
99-113
102-116
95-107
Average (%)
107
108
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-110
101
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
86-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.66
74.0
201.06
24.25
69.15
217.53
Standard deviation
3.0
6.76
18.82
2.32
5.38
21.82
C V (%)
12.17
9.14
9.36
9.57
7.78
10.03
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MIP-3β/ELC/CCL19 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MIP-3β/ELC/CCL19 in samples. No significant cross-reactivity or interference between Human MIP-3β/ELC/CCL19 and analogues was observed.
