Human MIP-1 beta (Macrophage Inflammatory Protein 1 Beta) CLIA Kit
The Human MIP-1 beta (Macrophage Inflammatory Protein-1 beta) CLIA Kit is designed for the accurate detection of MIP-1 beta levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.MIP-1 beta is a key chemokine involved in inflammatory responses and immune cell recruitment. It plays a crucial role in conditions such as inflammation, infection, and autoimmune diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.
Overall, the Human MIP-1 beta CLIA Kit from Assay Genie provides researchers with a powerful tool for investigating the role of MIP-1 beta in various physiological and pathological processes, ultimately advancing our understanding of the immune system and inflammatory pathways.
Product Name:
Human MIP-1 beta (Macrophage Inflammatory Protein 1 Beta) CLIA Kit
SKU:
HUES00022
Target:
Human MIP-1 beta (Macrophage Inflammatory Protein 1 Beta)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MIP-1β. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MIP-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MIP-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MIP-1β. You can calculate the concentration of Human MIP-1β in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-116
102-118
95-109
Average (%)
109
108
103
1:4
Range (%)
101-119
97-113
84-95
Average (%)
110
103
90
1:8
Range (%)
92-105
96-109
94-110
Average (%)
99
103
101
1:16
Range (%)
101-116
91-102
102-115
Average (%)
107
96
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
100-114
106
Cell culture media (n=5)
91-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.97
153.49
357.66
45.28
143.34
348.9
Standard deviation
4.87
18.27
30.26
5.48
15.84
23.93
C V (%)
10.37
11.9
8.46
12.1
11.05
6.86
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MIP-1β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MIP-1β in samples. No significant cross-reactivity or interference between Human MIP-1β and analogues was observed.
