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Human Milk Fat Globule EGF Factor 8 (MFGE8) ELISA Kit

SKU:
HUDL01836
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q08431
Sensitivity:
12.3pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
HMFG,MFGM,SED1,BA46,EDIL1,OAcGD3S,SPAG10,hP47,Medin,Lactadherin,Sperm Associated Antigen 10,Sperm Surface Protein hP47,Milk fat globule-EGF factor 8
Reactivity:
Human
Sample Type:
Serum, Plasma, Cell Lysates And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Milk Fat Globule EGF Factor 8 (MFGE8) ELISA Kit

The Human Milk Fat Globule EGF Factor 8 (MFGE8) ELISA Kit is designed for the accurate detection of MFGE8 levels in human milk samples. This kit offers high sensitivity and specificity, ensuring precise and reliable results for research and clinical applications.MFGE8, also known as lactadherin, is a protein involved in various biological processes, including immune regulation, inflammation, and tissue repair. It plays a crucial role in the maintenance of milk production and the development of the infant's immune system.

By accurately measuring MFGE8 levels in human milk, researchers can gain valuable insights into breastfeeding patterns, infant health, and potential therapeutic interventions. This ELISA kit provides a powerful tool for studying the role of MFGE8 in maternal-infant interactions and its impact on overall health outcomes.

Product Name:Human Milk Fat Globule EGF Factor 8 (MFGE8) ELISA Kit
Product Code:HUDL01836
Size:96 Assays
Target:MFGE8
Synonyms:HMFG, MFGM, SED1, BA46, EDIL1, OAcGD3S, SPAG10, hP47, Medin, Lactadherin, Sperm Associated Antigen 10, Sperm Surface Protein hP47, Milk fat globule-EGF factor 8
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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